Australian True Crime is hosted by Meshel Laurie, who conducts long-form interviews, typically 40 to 70 minutes, with people connected to Australian criminal cases. A recurring guest is Charlie Bezzina, a retired Victoria Police homicide detective who has appeared across multiple episodes discussing crime scene procedure, forensic reasoning, and internal police practice on cases ranging from staged burglaries to dismemberment. Other episodes feature family members of victims, such as Tracy Corbett Lynch and Sarah Corbett, discussing the long aftermath of a relative's murder years after it drew documentary or television coverage. Lawyers and advocates, including Andrew Carpenter and Adam Washbourne, also appear to discuss legal reform campaigns tied to child abuse compensation and offender asset seizure. The "ATC International" segment extends the same interview format to overseas cases with an Australian or Irish connection. Content leans heavily on procedural and forensic detail, including crime scene staging, entomology, and autopsy findings, alongside the emotional and legal aftermath for survivors and families. A number of episodes cover active legislative debates, such as proposed changes letting survivors access convicted offenders' superannuation. The tone is investigative and direct, with descriptions of violent death, sexual assault, and child abuse discussed without euphemism. Some episodes are labelled "Re-Issue," indicating the show republishes earlier interviews. The format suits listeners drawn to procedural and legal detail over dramatized narration.